| SES # | TOPICS | READINGS |
|---|---|---|
| 1 | General Introduction | |
| 2 | Discovery of Green Fluorescent Protein | Morise, H., O. Shimomura, F. H. Johnson, and J. Winant. "Intermolecular Energy Transfer in the Bioluminescent System of Aequorea." Biochemistry 13, no. 12 (June 4, 1974): 2656-62. Chalfie, M., Y. Tu, G. Euskirchen, W. W. Ward, and D. C. Prasher. "Green Fluorescent Protein as a Marker for Gene Expression." Science 263, no. 5148 (February 11, 1994): 802-5. |
| 3 | Fluorescent Protein Engineering | Yang, F., L. G. Moss, and G. N. Phillips, Jr. "The Molecular Structure of Green Fluorescent Protein." Nat Biotechnol 14, no. 10 (October 1996): 1246-51. Campbell, R. E., O. Tour, A. E. Palmer, P. A. Steinbach, G. S. Baird, D. A. Zacharias, and R. Y. Tsien. "A Monomeric Red Fluorescent Protein." Proc Natl Acad Sci U.S.A. 99, no. 12 (June 11, 2002): 7877-82. |
| 4 | Photoconversion of Fluorescent Proteins Variants to Probe Cellular Dynamics | Terskikh, A., A. Fradkov, G. Ermakova, A. Zaraisky, P. Tan, A. V. Kajava, X. Zhao, S. Lukyanov, M. Matz, S. Kim, I. Weissman, and P. Siebert. "Fluorescent Timer: Protein that Changes Color with Time." Science 290, no. 5496 (November 24, 2000): 1585-8. Patterson, G. H., and J. Lippincott-Schwartz. "A Photoactivatable GFP for Selective Photolabeling of Proteins and Cells." Science 297, no. 5588 (September 13, 2002): 1873-7. |
| 5 | Labeling Probes Other than Fluorescent Proteins | Griffin, B. A., S. R. Adams, and R. Y. Tsien. "Specific Covalent Labeling of Recombinant Protein Molecules Inside live Cells." Science 281, no. 5374 (July 10, 1998): 269-72. Juillerat, A., C. Heinis, I. Sielaff, J. Barnikow, H. Jaccard, B. Kunz, A. Terskikh, and K. Johnsson. "Engineering Substrate Specificity of O6-Alkylguanine-DNA Alkyltransferase for Specific Protein Labeling in Living Cells." Chembiochem 6, no. 7 (July 2005): 1263-9. |
| 6 | Visit to a Fluorescence Microscopy Facility | |
| 7 | Visualizing the Central Dogma of Molecular Biology | Janicki, S. M., T. Tsukamoto, S. E. Salghetti, W. P. Tansey, R. Sachidanandam, K. V. Prasanth, T. Ried, Y. Shav-Tal, E. Bertrand, R. H. Singer, and D. L. Spector. "From Silencing to Gene Expression: Real-Time Analysis in Single Cells." Cell 116, no. 5 (March 5, 2004): 683-98. Bratu, D. P., B. J. Cha, M. M. Mhlanga, F. R. Kramer, and S. Tyagi. "Visualizing the Distribution and Transport of mRNAs in Living Cells." U.S.A. Proc Natl Acad Sci 100, no. 23 (November 11, 2003): 13308-13. |
| 8 | Fluorescent Sensors of Cell Signaling: FRET | Miyawaki, A., J. Llopis, R. Heim, J. M. McCaffery, J. A. Adams, M. Ikura, and R. Y. Tsien. "Fluorescent Indicators for Ca2+ based on Green Fluorescent Proteins and Calmodulin." Nature 388, no. 6645 (August 28, 1997): 882-7. Reiff, D. F., A. Ihring, G. Guerrero, E. Y. Isacoff, M. Joesch, J. Nakai, and A. Borst. "In Vivo Performance of Genetically Encoded Indicators of Neural Activity in Flies." J Neurosci 25, no. 19 (May 11, 2005): 4766-78. |
| 9 | Quantitative and Ultra-Sensitive Fluorescent Imaging | Phair, R. D., and T. Misteli. "High Mobility of Proteins in the Mammalian Cell Nucleus." Nature 404, no. 6778 (April 6, 2000): 604-9. Tardin, C., L. Cognet, C. Bats, B. Lounis, and D. Choquet. "Direct Imaging of Lateral Movements of AMPA Receptors inside Synapses." EMBO J 22, no. 18 (September 15, 2003): 4656-65. |
| 10 | Student Presentations | |
| 11 | Power of Fluorescent Imaging for High-throughput Analysis | Wheeler, D. B., S. N. Bailey, D. A. Guertin, A. E. Carpenter, C. O. Higgins, and D. M. Sabatini. "RNAi Living-Cell Microarrays for Loss-Of-Function Screens in Drosophila Melanogaster Cells." Nat Methods 1, no. 2 (November 2004): 127-32. Epub (October 21, 2004). Neumann, B., M. Held, U. Liebel, H. Erfle, P. Rogers, R. Pepperkok, and J. Ellenberg. "High-Throughput RNAi Screening by Time-Lapse Imaging of Live Human Cells." Nat Methods 3, no. 5 (May 2006): 385-90. |
| 12 | Fluorescent Imaging in Living Organisms | Miller, M. J., S.H. Wei, I. Parker, and M. D. Cahalan. "Two-Photon Imaging of Lymphocyte Motility and Antigen Response in Intact Lymph Node." Science 296, no. 5574 (June 7, 2002): 1869-73. Amino, R., S. Thiberge, B. Martin, S. Celli, S. Shorte, F. Frischknecht, and R. Menard. "Quantitative Imaging of Plasmodium Transmission from Mosquito to Mammal." Nat Med 12, no. 2 (February 2006): 220-4. |