| SES # | TOPICS | readings |
|---|---|---|
| 1 | Introduction | |
| 2 | The Cell Cycle | Evans, T., E. T. Rosenthal, J. Youngblom, D. Distel, and T. Hunt. "Cyclin: A Protein Specified by Maternal mRNA in Sea Urchin Eggs that Is Destroyed at each Cleavage Division." Cell 33, no. 2 (1983): 389-396. (PDF)# Shirodkar, S., M. Ewen, J. A. DeCaprio, J. Morgan, D. M. Livingston, and T. Chittenden. "The Transcription Factor E2F Interacts with the Retinoblastoma Product and a p107-Cyclin A Complex in a Cell Cycle-Regulated Manner." Cell 68, no. 1 (1992): 157-166. |
| 3 | G1 Phase and Initiation of DNA Replication | Donovan, S., J. Harwood, L. S. Drury, and J. F. Diffley. "Cdc6p-Dependent Loading of Mcm Proteins onto Pre-Replicative Chromatin in Budding Yeast." Proceedings of the National Academy of Sciences of the United States of America 94, no. 11 (1997): 5611-5616. (PDF)# Mailand, N., and J. F. Diffley. "CDKs Promote DNA Replication Origin Licensing in Human Cells by Protecting Cdc6 from APC/C-Dependent Proteolysis." Cell 122, no. 6 (2005): 915-926. (PDF)# |
| 4 | S Phase and Replication Fork Regression | Dimitrova, D. S., and D. M. Gilbert. "The Spatial Position and Replication Timing of Chromosomal Domains Are both Established in Early G1 Phase." Molecular Cell 4, no. 6 (1999): 983-993. (PDF)# Moyer, S. E., P. W. Lewis, and M. R. Botchan. "Isolation of the Cdc45/Mcm2-7/GINS (CMG) Complex, a Candidate for the Eukaryotic DNA Replication Fork Helicase." Proceedings of the National Academy of Sciences of the United States of America 103, no. 27 (2006): 10236-10241. (PDF - 1.7 MB)# |
| 5 | Cell Cycle Control of DNA Replication | Hayles, J., D. Fisher, A. Woollard, and P. Nurse. "Temporal Order of S Phase and Mitosis in Fission Yeast Is Determined by the State of the p34cdc2 Mitotic B Cyclin Complex." Cell 78, no. 5 (1994): 813-822. Nguyen, V. Q., C. Co, and J. J. Li. "Cyclin-Dependent Kinases Prevent DNA Re-Replication through Multiple Mechanisms." Nature 411, no. 6841 (2001): 1068-1073. (PDF)# |
| 6 | Checkpoints | Kuerbitz, S. J., B. S. Plunkett, W. V. Walsh, and M. B. Kastan. "Wild-Type p53 Is a Cell Cycle Checkpoint Determinant Following Irradiation." Proceedings of the National Academy of Sciences of the United States of America 89, no. 16 (1992): 7491-7495. (PDF - 1.5 MB)# Katou, Y., Y. Kanoh, M. Bando, H. Noguchi, H. Tanaka, T. Ashikari, K.Sugimoto, and K. Shirahige. "S-Phase Checkpoint Proteins Tof1 and Mrc1 Form a Stable Replication-Pausing Complex." Nature 424, no. 6952 (2003): 1078-1083. |
| 7 | DNA Repair | Bienko, M., C. M. Green, N. Crosetto, F. Rudolf, G. Zapart, B. Coull, and P. Kannouche, et al. "Ubiquitin-Binding Domains in Y-Family Polymerases Regulate Translesion Synthesis." Science 310, no. 5755 (2005): 1821-1824. Spagnolo, L., A. Rivera-Calzada, L. H. Pearl, and O. Llorca. "Three Dimensional Structure of the Human DNA-PKcs/Ku70/Ku80 Complex Assembled on DNA and its Implications for DNA DSB Repair." Molecular Cell 22, no. 4 (2006): 511-519. |
| 8 | Midterm Assignments | |
| 9 | Development and Endoreduplication | Verkest, A., C. L. Manes, S. Vercruysse, S. Maes, E. Van Der Schueren, T. Beeckman, P. Genschik, M. Kuiper, D. Inze, and L. De Veylder. "The Cyclin-Dependent Kinase Inhibitor KRP2 Controls the Onset of the Endoreduplication Cycle during Arabidopsis Leaf Development through Inhibition of Mitotic CDKA; 1 Kinase Complexes." The Plant Cell 17, no. 6 (2005): 1723-1736. (PDF)# Gregoire, D., K. Brodolin, and M. Mechali. "HoxB Domain Induction Silences DNA Replication Origins in the Locus and Specifies a Single Origin at its Boundary." EMBO Reports 7, no. 8 (2006): 812-816. |
| 10 | Recombination and Meiosis | Borde, V., A. S. Goldman, and M. Lichten. "Direct Coupling between Meiotic DNA Replication and Recombination Initiation." Science 290, no. 5492 (2000): 806-809. Bhalla, N., and A. F. Dernburg. "A Conserved Checkpoint Monitors Meiotic Chromosome Synapsis in Caenorhabditis Elegans." Science 310, no. 5754 (2005): 1683-1686. |
| 11 | DNA Replication and Cancer | Tanaka, S. and J. F. Diffley. "Deregulated G1-Cyclin Expression Induces Genomic Instability by Preventing Efficient Pre-RC Formation." Genes & Development 16, no. 20 (2002): 2639-2649. Honeycutt, K. A., Z. Chen, M. I. Koster, M. Miers, J. Nuchtern, J. Hicks, D. R. Roop, and J. M. Shohet. "Deregulated Minichromosomal Maintenance Protein MCM7 Contributes to Oncogene Driven Tumorigenesis." Oncogene 25, no. 29 (2006): 4027-4032. |
| 12 | Targeting Replication Components as Chemotherapy | Li, J. L., Y. C. Cai, X. H. Liu, and L. J. Xian. "Norcantharidin Inhibits DNA Replication and Induces Apoptosis with the Cleavage of Initiation Protein Cdc6 in HL-60 Cells." Anti-Cancer Drugs 17, no. 3 (2006): 307-314. Cantero, G., N. Pastor, S. Mateos, C. Campanella, and F. Cortes. "Cisplatin-Induced Endoreduplication in CHO Cells: DNA Damage and Inhibition of Topoisomerase II." Mutation Research 599, no. 1-2 (2006): 160-166. |
| 13 | Viral Reprogramming of the Cell Cycle | Yew, P. R. and A. J. Berk. "Inhibition of p53 Transactivation Required for Transformation by Adenovirus Early 1B Protein." Nature 357, no. 6373 (1992): 82-85. Wiebusch, L., R. Uecker, and C. Hagemeier. "Human Cytomegalovirus Prevents Replication Licensing by Inhibiting MCM Loading Onto Chromatin." EMBO Reports 4, no. 1 (2003): 42-46. (PDF)# |
| 14 | Final Assignments Presented |